PARD6A 抗体 (C-Term)
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- 抗原 See all PARD6A 抗体
- PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PARD6A antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- 原理
- PAR6alpha / PARD6A
- 序列
- GSRIRGDGSG FSL
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.
- 亚型
- IgG
- Top Product
- Discover our top product PARD6A Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocar
Western Blot: Approx 40 kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4 kDa according to NP_058644.1) . Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))
- 别名
- PARD6A (PARD6A 产品)
- 别名
- PARD6A antibody, 0710008C04Rik antibody, 2610010A15Rik antibody, PAR6alpha antibody, Par-6 antibody, Par6 antibody, Par6c antibody, TAX40 antibody, Tip-40 antibody, Par-6a antibody, Par6a antibody, PAR-6A antibody, PAR6 antibody, PAR6C antibody, TIP-40 antibody, par-6 partitioning defective 6 homolog alpha (C. elegans) antibody, par-6 family cell polarity regulator alpha antibody, Partitioning defective protein 6 antibody, PARD6A antibody, Pard6a antibody, par-6 antibody
- 背景
- PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning d
- 基因ID
- 50855
- NCBI登录号
- NP_058644, NP_001032358
- 途径
- Cell-Cell Junction Organization
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