Pleckstrin 抗体 (C-Term)
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- 抗原 See all Pleckstrin (PLEK) 抗体
- Pleckstrin (PLEK)
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Pleckstrin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Pleckstrin
- 序列
- EWIKAIQMAS RTGK
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-EWIKAIQMASRTGK
- 亚型
- IgG
- Top Product
- Discover our top product PLEK Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 40-45 kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1 kDa according to Human NP_002655.2). Recommended concentration: 0.0
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- Pleckstrin (PLEK)
- 别名
- PLEK (PLEK 产品)
- 别名
- MGC69065 antibody, PLEK antibody, P47 antibody, 2010300B13Rik antibody, pleckstrin L homeolog antibody, pleckstrin antibody, plek.L antibody, PLEK antibody, Plek antibody
- 背景
- PLEK, pleckstrin, P47, p47, FLJ27168
- 基因ID
- 5341
- NCBI登录号
- NP_002655
- 途径
- Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
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