PPP1R8 抗体 (C-Term)
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- 抗原 See all PPP1R8 抗体
- PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PPP1R8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- NIPP1 / PPP1R8
- 序列
- EAWPGKKPTP SLLI
- 特异性
- This antibody is expected to recognise all three human isoforms of this protein.
- 交叉反应
- Cow, 犬, 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1.
- 亚型
- IgG
- Top Product
- Discover our top product PPP1R8 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8 μg/mL.
Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5 kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 h
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))
- 别名
- PPP1R8 (PPP1R8 产品)
- 别名
- ARD-1 antibody, ARD1 antibody, NIPP-1 antibody, NIPP1 antibody, PRO2047 antibody, 6330548N22Rik antibody, AU044684 antibody, PPP1R8 antibody, ard-1 antibody, ard1 antibody, nipp-1 antibody, nipp1 antibody, ppp1r8 antibody, si:ch211-206a7.1 antibody, protein phosphatase 1 regulatory subunit 8 antibody, protein phosphatase 1, regulatory subunit 8 antibody, protein phosphatase 1, regulatory (inhibitor) subunit 8 antibody, protein phosphatase 1, regulatory subunit 8b antibody, protein phosphatase 1 regulatory subunit 8 L homeolog antibody, protein phosphatase 1, regulatory subunit 8a antibody, protein phosphatase 1 regulatory subunit 8 S homeolog antibody, PPP1R8 antibody, Ppp1r8 antibody, ppp1r8b antibody, ppp1r8.L antibody, ppp1r8a antibody, ppp1r8.S antibody
- 背景
- PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory
- 基因ID
- 5511, 100336
- NCBI登录号
- NP_002704, NP_054829, NP_612568
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