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FOXA1 抗体 (C-Term)

Verified FOXA1 适用: 人, 小鼠 WB, IF, FACS, ELISA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185021
发货至: 中国
  • 抗原 See all FOXA1 抗体
    FOXA1 (Forkhead Box A1 (FOXA1))
    抗原表位
    • 27
    • 16
    • 12
    • 9
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 130
    • 65
    • 38
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 68
    • 62
    山羊
    克隆类型
    • 75
    • 55
    多克隆
    标记
    • 73
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FOXA1 antibody is un-conjugated
    应用范围
    • 92
    • 52
    • 49
    • 42
    • 35
    • 35
    • 21
    • 5
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA
    原理
    FOXA1 / HNF3A
    序列
    GVYSRPVLNT S
    交叉反应
    Cow, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
    亚型
    IgG
    Top Product
    Discover our top product FOXA1 Primary Antibody
  • 应用备注

    Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).

  • 抗原
    FOXA1 (Forkhead Box A1 (FOXA1))
    别名
    FOXA1 (FOXA1 产品)
    背景
    FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
    基因ID
    3169, 15375, 25098
    NCBI登录号
    NP_004487
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
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