TRIM5 抗体 (N-Term)
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- 抗原 See all TRIM5 抗体
- TRIM5 (Tripartite Motif Containing 5 (TRIM5))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This TRIM5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- TRIM5 / RNF88
- 序列
- ASGILVNVKE EVTC
- 特异性
- This antibody is expected to recognise all three human isoforms of this protein.
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3 μg/mL.
Western Blot: Approx 55 and 37 kDa bands observed in Human Thymus lysates (calculated MW of 56.3 kDa according to NP_149023.1 and of 37.7 kDa according to NP_149084.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- TRIM5 (Tripartite Motif Containing 5 (TRIM5))
- 别名
- TRIM5 (TRIM5 产品)
- 别名
- TRIM5 antibody, RNF88 antibody, TRIM5alpha antibody, EG667823 antibody, Gm8833 antibody, RGD1304579 antibody, tripartite motif containing 5 antibody, tripartite motif-containing 5 antibody, TRIM5 antibody, Trim5 antibody
- 背景
- TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
- 基因ID
- 85363
- NCBI登录号
- NP_149023, NP_149083, NP_149084
- 途径
- Activation of Innate immune Response
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