TRIM21 抗体 (C-Term)
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- 抗原 See all TRIM21 抗体
- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This TRIM21 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- SSA1 / Ro52
- 序列
- CPLNIGSQGS TDY
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.
- 亚型
- IgG
- Top Product
- Discover our top product TRIM21 Primary Antibody
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- 应用备注
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Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antib
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
- 别名
- TRIM21 (TRIM21 产品)
- 别名
- RNF81 antibody, RO52 antibody, SSA antibody, SSA1 antibody, Ro52 antibody, Ssa1 antibody, tripartite motif containing 21 antibody, tripartite motif-containing 21 antibody, TRIM21 antibody, Trim21 antibody
- 背景
- TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52 kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome ant
- 基因ID
- 6737
- NCBI登录号
- NP_003132
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