TRIM55 抗体 (C-Term)
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- 抗原 See all TRIM55 抗体
- TRIM55 (Tripartite Motif Containing 55 (TRIM55))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This TRIM55 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- MURF2 / TRIM55
- 序列
- HIFSFSWLNS LNE
- 特异性
- This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
- 交叉反应
- 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.
Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- TRIM55 (Tripartite Motif Containing 55 (TRIM55))
- 别名
- TRIM55 (TRIM55 产品)
- 别名
- MURF-2 antibody, RNF29 antibody, muRF2 antibody, trim55 antibody, wu:fb71c01 antibody, zgc:92123 antibody, TRIM55 antibody, im:7145460 antibody, zgc:136833 antibody, D830041C10Rik antibody, Murf2 antibody, Rnf29 antibody, murf-2 antibody, rnf29 antibody, tripartite motif containing 55 antibody, tripartite motif containing 55a antibody, tripartite motif containing 55b antibody, tripartite motif-containing 55 antibody, tripartite motif containing 55 L homeolog antibody, TRIM55 antibody, trim55a antibody, trim55b antibody, Trim55 antibody, trim55.L antibody
- 背景
- TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2
- 基因ID
- 84675, 381485, 365751
- NCBI登录号
- NP_908973, NP_908974, NP_908975
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