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TRIM55 抗体 (C-Term)

Verified TRIM55 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184953
发货至: 中国
  • 抗原 See all TRIM55 抗体
    TRIM55 (Tripartite Motif Containing 55 (TRIM55))
    抗原表位
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 19
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    宿主
    • 17
    • 2
    山羊
    克隆类型
    • 18
    • 1
    多克隆
    标记
    • 15
    • 2
    • 1
    • 1
    This TRIM55 antibody is un-conjugated
    应用范围
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    MURF2 / TRIM55
    序列
    HIFSFSWLNS LNE
    特异性
    This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
    交叉反应
    犬, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    TRIM55 (Tripartite Motif Containing 55 (TRIM55))
    别名
    TRIM55 (TRIM55 产品)
    别名
    MURF-2 antibody, RNF29 antibody, muRF2 antibody, trim55 antibody, wu:fb71c01 antibody, zgc:92123 antibody, TRIM55 antibody, im:7145460 antibody, zgc:136833 antibody, D830041C10Rik antibody, Murf2 antibody, Rnf29 antibody, murf-2 antibody, rnf29 antibody, tripartite motif containing 55 antibody, tripartite motif containing 55a antibody, tripartite motif containing 55b antibody, tripartite motif-containing 55 antibody, tripartite motif containing 55 L homeolog antibody, TRIM55 antibody, trim55a antibody, trim55b antibody, Trim55 antibody, trim55.L antibody
    背景
    TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2
    基因ID
    84675, 381485, 365751
    NCBI登录号
    NP_908973, NP_908974, NP_908975
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