PSMF1 抗体 (C-Term)
-
- 抗原 See all PSMF1 抗体
- PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))
-
抗原表位
- C-Term
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This PSMF1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 原理
- PI31 / PSMF1 (Isoform 1)
- 序列
- DHLPPPGYDD MYL
- 特异性
- This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-DHLPPPGYDDMYL
- 亚型
- IgG
-
-
- 应用备注
-
Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293, approx 30-32 kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35 kDa in lysates of cell line LNCaP (calculated MW of 29.8 kDa according to Human NP_006805.2 an
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry:A customer reported weak staining of A431 cells at
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))
- 别名
- PSMF1 (PSMF1 产品)
- 别名
- PI31 antibody, AW048666 antibody, BC012260 antibody, zgc:92785 antibody, proteasome inhibitor subunit 1 antibody, proteasome (prosome, macropain) inhibitor subunit 1 antibody, PSMF1 antibody, Psmf1 antibody, psmf1 antibody
- 背景
- PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1
- 基因ID
- 9491, 228769
- NCBI登录号
- NP_006805
- 途径
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
-