DPF1 抗体 (C-Term)
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- 抗原 See all DPF1 抗体
- DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DPF1 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF)
- 原理
- Neuro-d4 / DPF1
- 序列
- HLKEKASAYI TLT
- 特异性
- This antibody is expected to recognize all three reported isoforms (NP_001128627.1, NP_004638.2, NP_001128628.1).
- 交叉反应
- Cow, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1, NP_004638.2, NP_001128628.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Preliminary experiments showed a band at approx 38-40 kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1 μg/mL antibody staining (calculated MW of 37.9 kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:2000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))
- 别名
- DPF1 (DPF1 产品)
- 别名
- zgc:110104 antibody, DPF1 antibody, req antibody, xReq antibody, neud4 antibody, requiem antibody, neuro-d4 antibody, BAF45b antibody, NEUD4 antibody, Neud4 antibody, D4, zinc and double PHD fingers family 1 antibody, double PHD fingers 1 antibody, dpf1 antibody, DPF1 antibody, Dpf1 antibody
- 背景
- DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog
- 基因ID
- 8193, 29861, 50545
- NCBI登录号
- NP_001128627, NP_004638, NP_001128628
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