PUF60 抗体 (C-Term)
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- 抗原 See all PUF60 抗体
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PUF60 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 原理
- SIAHBP1 / FIR
- 序列
- YDQERFDNSD LSA
- 特异性
- This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1, NP_055096.2, NP_001129505.1)
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-YDQERFDNSDLSA, from the C Terminus of the protein sequence according to NP_510965.1, NP_055096.2, NP_001129505.1.
- 亚型
- IgG
- Top Product
- Discover our top product PUF60 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Prostate and Skin. Recommended concentration: 5 μg/mL.
Western Blot: Approx 60 kDa band observed in Human lysates of HEK293 (calculated MW of 59.9 kDa according to NP_510965.1 and 58.2 according to NP_055096.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
- 别名
- PUF60 (PUF60 产品)
- 别名
- FIR antibody, RoBPI antibody, SIAHBP1 antibody, fc21a05 antibody, wu:fc21a05 antibody, wu:fi43a08 antibody, 2410104I19Rik antibody, 2810454F19Rik antibody, Siahbp1 antibody, fe37c05 antibody, repressor antibody, si:ch211-12p8.2 antibody, si:zc12p8.2 antibody, wu:fb33e11 antibody, wu:fe37c05 antibody, zgc:86806 antibody, poly(U) binding splicing factor 60 antibody, poly-U binding splicing factor a antibody, poly-U binding splicing factor 60 antibody, poly-U binding splicing factor b antibody, PUF60 antibody, puf60a antibody, Puf60 antibody, puf60b antibody
- 背景
- PUF60, SIAHBP1, FIR, fuse-binding protein-interacting repressor, RoBPI, siah binding protein 1, poly-U binding splicing factor PUF60, Ro ribonucleoprotein-binding protein 1, pyrimidine tract binding splicing factor, poly-U binding splicing factor 60KDa, F
- 基因ID
- 22827, 67959
- NCBI登录号
- NP_510965, NP_055096, NP_001129505
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