ACSL4 抗体 (C-Term)
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- 抗原 See all ACSL4 抗体
- ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ACSL4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- FACL4 / ACSL4
- 序列
- HYLKDIERMY GGK
- 特异性
- This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HYLKDIERMYGGK, from the C Terminus of the protein sequence according to NP_004449.1, NP_075266.1.
- 亚型
- IgG
- Top Product
- Discover our top product ACSL4 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cortex). Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 80-85 kDa band observed in lysates of cell line HepG2 and approx 75 kDa in preliminary testing of Human Adipose lysate (calculated MW of 79.2 kDa according to NP_075266.1 and 74.4 kDa according to NP_004449.1). Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi and Endoplasmic reticulum of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))
- 别名
- ACSL4 (ACSL4 产品)
- 背景
- ACSL4, acyl-CoA synthetase long-chain family member 4, FACL4, fatty-acid-Coenzyme A ligase, long-chain 4, ACS4, LACS 4, lignoceroyl-CoA synthase, Fatty acid coenzyme A ligase, long-chain 4, MRX63, LACS4, MRX68, acyl-CoA synthetase 4, long-chain fatty-acid
- 基因ID
- 2182, 50790, 113976
- NCBI登录号
- NP_004449, NP_075266
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