UBE2C 抗体 (C-Term)
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- 抗原 See all UBE2C 抗体
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This UBE2C antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)
- 原理
- UBE2C / UBCH10
- 序列
- QETYSKQVTS QEP
- 特异性
- This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
- 亚型
- IgG
- Top Product
- Discover our top product UBE2C Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 70-179) antibody
UBE2C 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 9D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F5D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 25-129) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
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- 应用备注
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Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
- 别名
- UBE2C (UBE2C 产品)
- 别名
- LOC458287 antibody, UBCH10 antibody, dJ447F3.2 antibody, 1110015A16Rik antibody, D2Ertd695e antibody, im:7137135 antibody, zgc:123190 antibody, ubiquitin conjugating enzyme E2 C antibody, ubiquitin-conjugating enzyme E2C antibody, ubiquitin conjugating enzyme E2 C L homeolog antibody, ubiquitin conjugating enzyme E2 C S homeolog antibody, UBE2C antibody, NAEGRDRAFT_82946 antibody, ube2c.L antibody, Ube2c antibody, ube2c.S antibody, ube2c antibody
- 背景
- UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
- 基因ID
- 11065
- NCBI登录号
- NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670
- 途径
- Ubiquitin Proteasome Pathway
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