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ELF1 抗体 (C-Term)

Name: ELF1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN184790
Availability: OutOfStock
Rating: 5 (1 reviews)

Verified ELF1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

Western Blotting validation image for anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody (ABIN184790) (ELF1 抗体 (C-Term))

ABIN184790 staining (0. (ELF1 抗体 (C-Term))

IHC

5 images

(1 validation)

产品编号 ABIN184790

发货至: 中国

Datasheet as PDF

抗原 See all ELF1 抗体

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
抗原表位
15

4

4

4

2

1

1

1

1

1

1

1
C-Term
适用
44

7

6

6

4

3

2

2

1

1

1

1
人, 小鼠
宿主
41

3
山羊
Compatible Secondaries
克隆类型
43

1
多克隆
标记
24

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ELF1 antibody is un-conjugated
应用范围
35

13

13

10

10

6

6

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

原理

ELF1

序列

AMKQNELLEP NSF

特异性

This antibody is expected to recognise both reported isoforms.

交叉反应

人, 小鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-AMKQNELLEPNSF, from the C Terminus of the protein sequence according to NP_758961.1, NP_001138825.1.

亚型

IgG
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody
ELF1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (N-Term) antibody
ELF1 适用: 人, 小鼠, 兔, 大鼠, 马, 犬 WB 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-210) antibody
ELF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 421-520) antibody
ELF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B7 unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibody
ELF1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 142-191) antibody
ELF1 适用: 人, 兔, 大鼠, 马, 犬, Cow, 豚鼠, Bat, Hamster, 猴, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody
ELF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibody
ELF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 540-589) antibody
ELF1 适用: 人, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated

anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 131-180) antibody
ELF1 适用: 人, 小鼠, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 65 kDa band observed in Human Lymph Node and Tonsil and in Mouse Thymus lysates (calculated MW of 64.9 kDa according to Human NP_001138825.1 and 62.3 kDa according to Mouse NP_001273341.1). Recommended concentration: 0.1-0.3 μg/mL. Appro
Peptide ELISA: antibody detection limit dilution 1:64000.

限制

仅限研究用
生效 #103876 (Western Blotting)

by

Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine

No.

#103876

日期

2019.10.28

抗原

ELF1

Lot Number

C2P2

Method validated

Western Blotting

Positive Control

A549 (endogenous ELF1), ELF1 recombinant protein

Negative Control

Notes

Passed. ABIN184790 specifically recognizes endogenous ELF1 in A549 cell lysates and recombinant human ELF1.

Validation Images

Detection of human ELF1 by western blot using ABIN184790 diluted 1:2500. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 65kDa.
Full Methods
Primary Antibody

ABIN184790

Secondary Antibody

rabbit anti-goat HRP-conjugated antibody (Invitrogen, 31402)

Full Protocol

Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO₂ to 3x10⁴ cells/cm² in 2ml on a 6 well plate (Costar, 3516).
Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
Take cells up in growth medium.
Wash cells once with 1x PBS.
Resuspend approximately 2.05x10⁵cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
Denature samples for 3min at 95°C and subsequently keep them on ice.
Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
Incubation with primary
goat anti-ELF1 antibody (antibodies-online, ABIN184790, C2P2) diluted 1:2500 in TBST containing 0.5% skim milk ON at 4°C.
loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
Wash membrane 3x for 10min with TBST.
Incubation with secondary
rabbit anti-goat HRP-conjugated antibody (Invitrogen, 31402) diluted 1:20000 in TBST containing 0.5% skim milk for 1h at RT.
goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
Wash membrane 3x for 10min with TBST.
Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).

Experimental Notes

ABIN184790 reveals a protein with an apparent molecular weight of approximately 62kDa. The expected molecular weight for endogenous human ELF1 is approximately 67kDa. The antibody also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).
Other ELF1-antibody dilutions were tested, but a dilution of 1:2500 was found optimal in terms of minimal background and strength of signal.
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))

别名

ELF1 (ELF1 产品)

别名

zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody

背景

ELF1, E74-like factor 1 (ets domain transcription factor), OTTHUMP00000018310, ets-related transcription factor Elf-1

基因ID

1997, 13709

NCBI登录号

NP_758961, NP_001138825