AIRE 抗体 (C-Term)
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- 抗原 See all AIRE 抗体
- AIRE (Autoimmune Regulator (AIRE))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This AIRE antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- 原理
- AIRE
- 序列
- QSMARPAAPF PS
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.
- 亚型
- IgG
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anti-Autoimmune Regulator (AIRE) (AA 30-130) antibody
AIRE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (N-Term) antibodyAIRE 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (Internal Region) antibodyAIRE 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (Internal Region) antibodyVerified AIRE 适用: 人, Pig WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (AA 39-108) antibodyAIRE 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (pSer156) antibodyAIRE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (AA 467-516) antibodyAIRE 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (AA 218-267) antibodyAIRE 适用: 人, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) antibodyAIRE 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Autoimmune Regulator (AIRE) (Internal Region) antibodyAIRE 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL.
Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. R
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- AIRE (Autoimmune Regulator (AIRE))
- 别名
- AIRE (AIRE 产品)
- 别名
- AIRE1 antibody, APECED antibody, APS1 antibody, APSI antibody, PGA1 antibody, autoimmune regulator antibody, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, AaeL_AAEL013794 antibody, CpipJ_CPIJ001208 antibody, CpipJ_CPIJ001209 antibody, AIRE antibody, Aire antibody
- 背景
- AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop
- 基因ID
- 326
- NCBI登录号
- NP_000374
- 途径
- Chromatin Binding
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