KIF5B 抗体 (C-Term)
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- 抗原 See all KIF5B 抗体
- KIF5B (Kinesin Family Member 5B (KIF5B))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This KIF5B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 原理
- Kinesin 1 / UKHC
- 序列
- QPVAVRGGGG KQV
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
- 亚型
- IgG
- Top Product
- Discover our top product KIF5B Primary Antibody
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- 应用备注
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Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).
: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).
: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).
: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).
: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).
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ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).
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- 抗原
- KIF5B (Kinesin Family Member 5B (KIF5B))
- 别名
- KIF5B (KIF5B 产品)
- 别名
- KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, Khc antibody, AL022807 antibody, Khcs antibody, Kns1 antibody, Ukhc antibody, khc antibody, KIF5A antibody, kinesin family member 5B antibody, kinesin heavy chain antibody, kinesin-1 antibody, kinesin-1 heavy chain antibody, kinesin family member 5B S homeolog antibody, KIF5B antibody, LOC411769 antibody, cgd6_2790 antibody, kin-1 antibody, Tb927.1.1350 antibody, LACBIDRAFT_305999 antibody, Khc antibody, LOC100538629 antibody, Kif5b antibody, kif5b.S antibody
- 背景
- KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
- 基因ID
- 3799
- NCBI登录号
- NP_004512
- 途径
- Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization
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