KLF3 抗体 (N-Term)
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- 抗原 See all KLF3 抗体
- KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This KLF3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)
- 原理
- KLF3 / BKLF
- 序列
- LMFDPVPVKQ EAMD
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence LMFDPVPVKQEAMD-C, from the N Terminus of the protein sequence according to NP_057615.3.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 38-40 kDa band observed in Human Heart lysates (predicted MW of 38.8Da according to NP_057615.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))
- 别名
- KLF3 (KLF3 产品)
- 别名
- BKLF antibody, 9930027G08Rik antibody, AL024007 antibody, Bklf antibody, Tef-2 antibody, klf12 antibody, Kruppel like factor 3 antibody, Kruppel-like factor 3 (basic) antibody, KLF3 antibody, Klf3 antibody, klf3 antibody
- 背景
- KLF3, BKLF, Kruppel-like factor 3 (basic), basic kruppel like factor, MGC48279
- 基因ID
- 51274
- NCBI登录号
- NP_057615
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