APEX1 抗体 (N-Term)
-
- 抗原 See all APEX1 抗体
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
-
抗原表位
- N-Term
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This APEX1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- APE1 / APEX1
- 序列
- PKRGKKGAVA EDGD
- 特异性
- Reported variants represent identical protein (NP_001632.2, NP_542379.1, NP_542380.1).
- 交叉反应
- Cow, 犬, 人, Pig
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2.
- 亚型
- IgG
- Top Product
- Discover our top product APEX1 Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 37 kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6 kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
-
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 别名
- APEX1 (APEX1 产品)
- 别名
- apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody
- 背景
- APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, a
- 基因ID
- 328
- NCBI登录号
- NP_001632
- 途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
-