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FOXP3 抗体 (C-Term)

Verified FOXP3 适用: 人 WB, FACS, ELISA, IHC, IF, ICC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184677
发货至: 中国
  • 抗原 See all FOXP3 抗体
    FOXP3 (Forkhead Box P3 (FOXP3))
    抗原表位
    • 22
    • 16
    • 15
    • 9
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    • 6
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    C-Term
    适用
    • 153
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    宿主
    • 112
    • 59
    • 16
    • 1
    山羊
    克隆类型
    • 112
    • 76
    多克隆
    标记
    • 86
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    This FOXP3 antibody is un-conjugated
    应用范围
    • 126
    • 79
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    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    原理
    FOXP3 / SCURFIN
    序列
    SQRPSRCSNP TPGP
    特异性
    This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.
    亚型
    IgG
    Top Product
    Discover our top product FOXP3 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    FOXP3 (Forkhead Box P3 (FOXP3))
    别名
    FOXP3 (FOXP3 产品)
    别名
    foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody
    背景
    FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
    基因ID
    50943
    NCBI登录号
    NP_054728, NP_001107849
    途径
    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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