FOXP3 抗体 (C-Term)
-
- 抗原 See all FOXP3 抗体
- FOXP3 (Forkhead Box P3 (FOXP3))
-
抗原表位
- C-Term
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This FOXP3 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 原理
- FOXP3 / SCURFIN
- 序列
- SQRPSRCSNP TPGP
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.
- 亚型
- IgG
- Top Product
- Discover our top product FOXP3 Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- FOXP3 (Forkhead Box P3 (FOXP3))
- 别名
- FOXP3 (FOXP3 产品)
- 别名
- foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody
- 背景
- FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
- 基因ID
- 50943
- NCBI登录号
- NP_054728, NP_001107849
- 途径
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
-