SIAH1 抗体 (N-Term)
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- 抗原 See all SIAH1 抗体
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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抗原表位
- N-Term
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SIAH1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 原理
- SIAH1
- 序列
- SRQTATALPT GTSKC
- 特异性
- This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
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BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
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- 抗原
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
- 别名
- SIAH1 (SIAH1 产品)
- 别名
- SIAH antibody, Siah1 antibody, Siah1a antibody, id:ibd2635 antibody, zgc:56026 antibody, SIAH1A antibody, siah antibody, E3 ubiquitin-protein ligase Siah1 antibody, E3 ubiquitin-protein ligase siah-1 antibody, siah E3 ubiquitin protein ligase 1 antibody, siah E3 ubiquitin protein ligase 1 L homeolog antibody, Tsp_03055 antibody, siah-1 antibody, Siah1 antibody, siah1 antibody, SIAH1 antibody, siah1.L antibody
- 背景
- SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog
- 分子量
- 31.1kDa
- 基因ID
- 6477, 20437, 140941
- NCBI登录号
- NP_003022, NP_001006611
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