PYCARD 抗体 (C-Term)
-
- 抗原 See all PYCARD 抗体
- PYCARD (PYD and CARD Domain Containing (PYCARD))
-
抗原表位
- C-Term
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This PYCARD antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- ASC / TMS1
- 序列
- RESQSYLVED LERS
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.
- 亚型
- IgG
- Top Product
- Discover our top product PYCARD Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 19 kDa band observed in lysates of cell line HeLa (calculated MW of 20.0 kDa according to NP_660183.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- PYCARD (PYD and CARD Domain Containing (PYCARD))
- 别名
- PYCARD (PYCARD 产品)
- 别名
- ASC antibody, CARD5 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, 9130417A21Rik antibody, Asc antibody, TNS1 antibody, masc antibody, asc1 antibody, zAsc antibody, zgc:109874 antibody, PYD and CARD domain containing antibody, PYCARD antibody, Pycard antibody, pycard antibody
- 背景
- PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1
- 基因ID
- 29108
- NCBI登录号
- NP_037390, NP_660183
- 途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
-