GRB2 抗体 (C-Term)
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- 抗原 See all GRB2 抗体
- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GRB2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- GRB2
- 序列
- PRNYVTPVNR NV
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.
- 亚型
- IgG
- Top Product
- Discover our top product GRB2 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 26 kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2 kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
- 别名
- GRB2 (GRB2 产品)
- 别名
- GRB2 antibody, grb2 antibody, AA408164 antibody, Ash antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, wu:fb18e12 antibody, wu:fb34e06 antibody, zgc:73077 antibody, growth factor receptor bound protein 2 antibody, growth factor receptor-bound protein 2 antibody, growth factor receptor-bound protein 2b antibody, GRB2 antibody, grb2 antibody, Grb2 antibody, grb2b antibody
- 背景
- GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096,
- 基因ID
- 2885, 14784, 81504
- NCBI登录号
- NP_002077, NP_987102
- 途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
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