NFIL3 抗体 (C-Term)
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- 抗原 See all NFIL3 抗体
- NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This NFIL3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 原理
- NFIL3
- 序列
- RLIATQPISA SDSG
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RLIATQPISASDSG, from the C Terminus of the protein sequence according to NP_005375.2.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx. 60 kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5 kDa according to NP_005375.2). This molecular weight is routinely observed by other sources. An additional band of 30 kDa was observed in lysat
Peptide ELISA: antibody detection limit dilution 1:2000.
- 说明
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Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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