ITK 抗体 (C-Term)
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- 抗原 See all ITK 抗体
- ITK (IL2-Inducible T-Cell Kinase (ITK))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ITK antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 原理
- ITK
- 序列
- RLLRQLAEIA ESGL
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.
- 亚型
- IgG
- Top Product
- Discover our top product ITK Primary Antibody
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- 应用备注
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Western Blot: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:2000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.
Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentrat
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- ITK (IL2-Inducible T-Cell Kinase (ITK))
- 别名
- ITK (ITK 产品)
- 别名
- Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody
- 背景
- ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
- 基因ID
- 3702, 16428
- NCBI登录号
- NP_005537
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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