B-Cell Linker 抗体 (C-Term)
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- 抗原 See all B-Cell Linker (BLNK) 抗体
- B-Cell Linker (BLNK)
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This B-Cell Linker antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- BLNK / SLP-65
- 序列
- KDSTRLKYAV KVS
- 特异性
- This antibody is expected to recognize all reported isoforms.
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.
- 亚型
- IgG
- Top Product
- Discover our top product BLNK Primary Antibody
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- 应用备注
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Western Blot: Approx 70-75 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour a
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- B-Cell Linker (BLNK)
- 别名
- BLNK (BLNK 产品)
- 别名
- BLNK antibody, blnk antibody, MGC147045 antibody, BASH antibody, Bca antibody, Ly-57 antibody, Ly57 antibody, Lyw-57 antibody, SLP-65 antibody, AGM4 antibody, BLNK-S antibody, LY57 antibody, SLP65 antibody, bca antibody, B-cell linker antibody, B cell linker antibody, BLNK antibody, blnk antibody, Blnk antibody
- 背景
- BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leuko
- 基因ID
- 29760, 17060
- NCBI登录号
- NP_037446, NP_001107566, NP_001245369, NP_001245370, NP_001245371
- 途径
- BCR Signaling
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