MAX 抗体 (C-Term)
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- 抗原 See all MAX 抗体
- MAX (MYC Associated Factor X (MAX))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This MAX antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- MAX
- 序列
- EEPQSRKKLR MEAS
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_002373.3, NP_660087.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EEPQSRKKLRMEAS, from the C Terminus of the protein sequence according to NP_002373.3, NP_660087.1.
- 亚型
- IgG
- Top Product
- Discover our top product MAX Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.
Western Blot: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10μg/ml.
Additional validation: This antibody has been successfully used in the followin
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- MAX (MYC Associated Factor X (MAX))
- 别名
- MAX (MAX 产品)
- 背景
- MAX, MAX protein, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, myc-associated factor X, helix-loop-helix zipper protein, MYC associated factor X, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1
- 基因ID
- 4149, 17187
- NCBI登录号
- NP_002373, NP_660087
- 途径
- Mitotic G1-G1/S Phases
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