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CBP Tag 抗体

WB, ELISA 宿主: 小鼠 Monoclonal 1D11E5 unconjugated
产品编号 ABIN1842584
发货至: 中国
  • 抗原 See all CBP Tag products
    CBP Tag
    适用
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    宿主
    • 16
    • 7
    小鼠
    克隆类型
    • 16
    • 6
    单克隆
    标记
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    This CBP Tag antibody is un-conjugated
    应用范围
    • 13
    • 13
    • 9
    • 8
    • 3
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    • 1
    • 1
    Western Blotting (WB), ELISA
    序列
    KRRWKKNFIA VSAA
    特异性
    CBP Tag Antibody, mAb, Mouse recognizes CBP-tagged proteins.
    产品特性
    CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.
    纯化方法
    Protein A affinity column
    免疫原
    CBP tag peptide conjugated to KLH
    克隆位点
    1D11E5
    亚型
    IgG2b kappa
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    Western blot: 1 µg/mL.
    Immunoprecipitation: 1-5 µg/mL.
    ELISA: 0. 001-0. 002 µg/mL.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    浓度
    0.5 mg/mL
    缓冲液
    lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
  • Li, Liu, Zhang, Issaian, Hill, Espinosa, Shi, Cui, Kappel, Das, Hansen, Zhou, Zhao: "A unified mechanism for intron and exon definition and back-splicing." in: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).

  • 抗原
    CBP Tag
    Abstract
    CBP Tag 产品
    物质类
    Tag
    背景
    CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins
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