Anti-E-tag [HRP] Polyclonal Antibody recognizes E tagged fusion proteins.
产品特性
Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.
纯化方法
Immunoaffinity chromatography
免疫原
E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL Western blot: 0. 1-1. 0 µg/mL Other applications: user-optimized
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
浓度
0.5 mg/mL
缓冲液
lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.