The PEG Antibody [Biotin], mAb, Mouse detects PEG backbone. The product can detect the variety of PEGs such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.
Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA Detector: 0. 1-1. 0 µg/mL Western Blot: 0. 1-1. 0 µg/mL ELISA Capturer: customer optimized Competitive ELISA: customer optimized Double Antigen Bridging ELISA: customer optimized
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL. For long-term storage, we suggest adding glycerol to 50 % to a reconstituted antibody.
浓度
0.5 mg/mL
缓冲液
lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
The PEG Antibody [Biotin], mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20 °C or below. The reconstituted product can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalently couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs. PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Biotin conjugated PEG Antibody is a useful tool for the detection of PEGylated molecules. The PEG Antibody [Biotin], mAb, Mouse is PEG antibody conjugated with biotin.