This antibody recognizes the Integrin beta 1 subunit (CD29). Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. (Ref.1) We recommend the use of SM2210LE for this purpose.
纯化方法
Affinity Chromatography on Protein G.
免疫原
Purified mouse VLA-4 antigen. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Flow Cytometry (Neat-1/5): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.25 mL distilled water.
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
This product is photosensitive and should be protected from light
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12