His Tag 抗体 (FITC)
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- 抗原 See all His Tag 抗体
- His Tag
- 适用
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This His Tag antibody is conjugated to FITC
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS)
- 序列
- HHHHHH
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- 克隆位点
- AD1-1-10
- 亚型
- IgG1
- Top Product
- Discover our top product His Tag Primary Antibody
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- His Tag
- 别名
- 6xHistidine Epitope Tag (His Tag 产品)
- 物质类
- Tag
- 背景
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
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