CD4 抗体
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- 抗原 See all CD4 抗体
- CD4
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- 克隆位点
- RPA-T4
- 亚型
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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- 应用备注
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Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended Positive Control: Human lymphatic tissue
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
- CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- 基因ID
- 920
- NCBI登录号
- NP_000607
- UniProt
- P01730
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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