ELISA: Indirect: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Sandwich: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-MurineSF-20 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mSF-20. Western blot: To detect mSF-20 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
C19orf10
(Chromosome 19 Open Reading Frame 10 (C19orf10))