TGFB1 抗体
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- 抗原 See all TGFB1 抗体
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TGFB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects TGF-3.
- 产品特性
- Synonyms: Transforming growth factor beta-1, TGFB, LAP, Latency-associated peptide
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Highly pure (>98%) recombinant Human TGFB1
- 亚型
- IgG1
- Top Product
- Discover our top product TGFB1 Primary Antibody
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- 应用备注
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ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human TGFB1 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be usedat a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, undernon-reducing conditions. In a dot blot, a 1/500 dilution of this antibody exhibited complete cross reactivity withHuman TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
- 缓冲液
- PBS without preservatives
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
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The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 别名
- TGFB1 (TGFB1 产品)
- 背景
- Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1
- 基因ID
- 7040
- UniProt
- P01137
- 途径
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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