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Nestin 抗体 (FITC)

NES 适用: 人 IF, ICC 宿主: 小鼠 Monoclonal 10C2 FITC
产品编号 ABIN1774766
发货至: 中国
  • 抗原 See all Nestin (NES) 抗体
    Nestin (NES)
    适用
    • 136
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    宿主
    • 109
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    • 1
    • 1
    小鼠
    克隆类型
    • 114
    • 35
    单克隆
    标记
    • 62
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    This Nestin antibody is conjugated to FITC
    应用范围
    • 74
    • 48
    • 48
    • 48
    • 41
    • 39
    • 34
    • 27
    • 24
    • 16
    • 9
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    Immunofluorescence (IF), Immunocytochemistry (ICC)
    特异性
    This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
    纯化方法
    Protein G purified
    免疫原
    A 150-amino-acid fragment from the cloned human nestin conjugated to GST
    克隆位点
    10C2
    亚型
    IgG1
    Top Product
    Discover our top product NES Primary Antibody
  • 应用备注
    Optimal antibody dilution should be determined by titration.
    限制
    仅限研究用
  • 生效 #029824 (Immunofluorescence)
    '独立验证'标志
    by
    Ensigna Biosystems
    No.
    #029824
    日期
    2014.11.06
    抗原
    Lot Number
    I030314
    Method validated
    Immunofluorescence
    Positive Control
    PC-3 cells
    Negative Control
    MCF-7 cells
    Notes
    Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Nestin (NES) (FITC)
    • Catalog number: ABIN1774766
    • Lot number: I030314
    • Dilution: 1:75, 1:100
    Secondary Antibody
    Full Protocol
    • Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
    • Cells were rinsed two times in 1X PBS.
    • Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with DAPI-Fluoromount-G.
    • Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
    Experimental Notes
    The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    Nestin (NES)
    别名
    Nestin (NES 产品)
    别名
    paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
    背景
    Neural Stem Cell Marker
    基因ID
    407242
    OMIM
    600915
    UniProt
    P18175
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