Tubulin delta 2 (AA 443-449) 抗体
适用: 人, 哺乳动物, 小鼠, 大鼠
WB, ICC
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Tubulin delta 2
- 抗原表位
- AA 443-449
- 适用
- 人, 哺乳动物, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- Specific for delta2-tubulin.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
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anti-Tubulin delta 2 (C-Term) antibody
适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet - 说明
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This antibody has been cross-adsorbed against peptides corresponding to the C-termini of glu- and tyr-tubulin. ICC: Requires long permeabilization with 0.3% Tritonx100 for 30 min.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- 抗原
- Tubulin delta 2
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