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alpha Tubulin 抗体 (AA 419-435)

Name: alpha Tubulin 抗体 (AA 419-435)
SKU: ABIN1742547
Availability: OutOfStock

TUBA1 适用: 人, 小鼠, 大鼠, Saccharomyces cerevisiae, Vertebrate, Invertebrate WB, ELISA, IP, ICC 宿主: 小鼠 Monoclonal 3A2 unconjugated

dilution: 1 : 1000, sample: rat brain homogenate (alpha Tubulin 抗体 (AA 419-435))

Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). (alpha Tubulin 抗体 (AA 419-435))

IHC

Immunocytochemistry (ICC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) (alpha Tubulin 抗体 (AA 419-435))

ICC

Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution 1 : 500; green). (alpha Tubulin 抗体 (AA 419-435))

IHC (p)

4 images

(3 references)

产品编号 ABIN1742547

发货至: 中国

Datasheet as PDF

抗原 See all alpha Tubulin (TUBA1) 抗体

alpha Tubulin (TUBA1)
抗原表位
16

11

6

5

4

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 419-435
适用
102

76

67

22

19

16

14

11

8

5

5

4

4

4

4

4

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, Saccharomyces cerevisiae, Vertebrate, Invertebrate
宿主
56

52

8

2

2

1

1

1
小鼠
Compatible Secondaries
克隆类型
68

52

2
单克隆
标记
85

11

6

3

2

2

2

2

2

2

2

1

1

1

1
This alpha Tubulin antibody is un-conjugated
应用范围
97

44

38

35

34

33

25

15

7

7

4

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

纯化方法

purified IgG. Azide was added before lyophilization.

免疫原

Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

克隆位点

3A2

亚型

IgG1

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anti-alpha Tubulin (TUBA1) antibody
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应用备注

WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200

说明

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

限制

仅限研究用
状态

Lyophilized

溶解方式

For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

缓冲液

PBS, 0.02% sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

储存条件

-20 °C

储存方法

Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).
抗原

alpha Tubulin (TUBA1)

别名

alpha-Tubulin (TUBA1 产品)

别名

Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

途径

Microtubule Dynamics