Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
特异性
Recognizes GalphaO 1 and GalphaO 2
纯化方法
purified IgG
免疫原
Recombinant full length rodent GalphaO 2.
克隆位点
101-1
亚型
IgG2b
应用备注
WB: 1 : 1000 (AP staining) ICC: 1 : 500 up to 1 : 1000 IHC: not tested yet
限制
仅限研究用
状态
Lyophilized
溶解方式
For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
缓冲液
PBS
注意事项
Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
储存条件
-20 °C
储存方法
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).
Winter, Brunk, Walther, Höltje, Jiang, Peter, Takamori, Jahn, Birnbaumer, Ahnert-Hilger: "Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 18, pp. 4672-80, (2005) (PubMed).