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- 抗原 See all TNC 抗体
- TNC (Tenascin C (TNC))
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适用
- 大鼠, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This TNC antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Specific for tenascin-C splice variants carrying the FNIII D domain.
- 纯化方法
- hybridoma supernatant
- 免疫原
- Glycopeptidase F-treated tenascin from mouse brain. Epitope FNIII D domain
- 克隆位点
- 578
- 亚型
- IgG
- Top Product
- Discover our top product TNC Primary Antibody
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- 应用备注
- 1:10.000 recommended dilution
- 说明
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Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
- 缓冲液
- PBS
- 注意事项
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When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation. - 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).
: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, (2002) (PubMed).
: "Expression of tenascin in the developing and adult cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, (1992) (PubMed).
: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, (1990) (PubMed).
: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, (1989) (PubMed).
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The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).
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- 抗原
- TNC (Tenascin C (TNC))
- 别名
- Tenascin-C (TNC 产品)
- 别名
- tn antibody, 150-225 antibody, GMEM antibody, GP antibody, HXB antibody, JI antibody, TN antibody, TN-C antibody, AI528729 antibody, C130033P17Rik antibody, Hxb antibody, Ten antibody, cytotactin antibody, tenascin-C antibody, P230 antibody, tenc antibody, wu:fk04d02 antibody, tenascin C L homeolog antibody, tenascin C antibody, tnc.L antibody, TNC antibody, MCYG_00667 antibody, tnc antibody, Tnc antibody
- 背景
- Synonyms: TN-C, J1-200/220, Cytoactin
- 途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
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