Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
免疫原
Purified recombinant protein (aa 152-354 of human homer1b)
应用备注
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500 IHC: not tested yet
限制
仅限研究用
状态
Lyophilized
溶解方式
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
缓冲液
PBS
注意事项
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
储存条件
-20 °C
储存方法
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Yoon, Piguel, Khalatyan, Dionisio, Savas, Penzes: "Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome." in: Molecular psychiatry, (2021) (PubMed).
Kim, Kong, Yamamoto, Kuramoto, Huang, Wang, Hong, Xiao, Levine, Qiu, Zhao, Miller, Dong, Meltzer, Xu, He: "An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system." in: Science advances, Vol. 7, Issue 8, (2021) (PubMed).
Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).