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GFP 抗体

Name: GFP 抗体
SKU: ABIN1742293
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ICC 宿主: 兔 Polyclonal unconjugated

dilution: 1 : 1000, sample: total cell lysate of GFP transfected fibroblasts (GFP 抗体)

Image no. 1 for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293) (GFP 抗体)

2 images

(1 reference)

产品编号 ABIN1742293

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
175

15

11

9
Aequorea victoria
宿主
64

63

34

13

9

5

2

1
兔
Compatible Secondaries
克隆类型
113

73
多克隆
标记
82

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
148

80

47

34

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC)

交叉反应（详细）

Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

纯化方法

Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

免疫原

Strep-Tag® fusion protein, full-length GFP.

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应用备注

WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

说明

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

限制

仅限研究用
状态

Lyophilized

溶解方式

For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

缓冲液

PBS

注意事项

Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

储存条件

-20 °C

储存方法

Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Rotmann, Strand, Martiné, Closs: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody