SV2B 抗体 (AA 2-17)
-
- 抗原 See all SV2B 抗体
- SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))
-
抗原表位
- AA 2-17
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This SV2B antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- 特异性
- Specific for SV2 B.
- 纯化方法
- purified IgG
- 免疫原
- Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
- 克隆位点
- 246E8
- 亚型
- IgG2b
- Top Product
- Discover our top product SV2B Primary Antibody
-
-
- 应用备注
-
WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200
IHC-P: 1 : 500 - 说明
-
WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS, 0.02% sodium azide (= 0.02 µg)
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
-
-
BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).
: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).
: "
-
BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).
-
- 抗原
- SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))
- 别名
- SV2 B (SV2B 产品)
- 别名
- SV2B antibody, A830038F04Rik antibody, AI850768 antibody, mKIAA0735 antibody, HsT19680 antibody, synaptic vesicle glycoprotein 2B antibody, synaptic vesicle glycoprotein 2 b antibody, synaptic vesicle glycoprotein 2b antibody, SV2B antibody, CpipJ_CPIJ009611 antibody, Sv2b antibody
-