Clathrin 抗体 (AA 156-173)
适用: 人, 小鼠, 大鼠, 斑马鱼
IHC, WB, ICC
宿主: 小鼠
Monoclonal
57-4
unconjugated
(11 references)-
- 抗原 See all Clathrin products
- Clathrin
- 抗原表位
- AA 156-173
- 适用
- 人, 小鼠, 大鼠, 斑马鱼
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Clathrin antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- Specific for both neuronal light chains
- 交叉反应 （详细）
- does not cross-react with the non-neuronal variants.
- 纯化方法
- purified IgG
- 免疫原
- Synthetic peptide IADKAFYQQPDADTIGYV (aa 156-173) corresponding to the spliced insert of the neuronal light chain variants.
- 克隆位点
- 57-4
- 亚型
- IgG1
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- 应用备注
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WB: 1 : 5000 up to 1 : 10000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC-P: 1 : 2000 - 说明
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Suitable for immunogold electron microscopy.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).
: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Role for Reelin in neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).
: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).
: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).
: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." in: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).
: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).
: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).
: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).
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: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).
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- 抗原
- Clathrin
- Abstract
- Clathrin 产品
- 别名
- CG9012 antibody, CHC antibody, CLH antibody, Cla antibody, D-Chc antibody, Dmel\\CG9012 antibody, chc antibody, dCHC antibody, l(1)13Fb antibody, l(1)G0438 antibody, l(1)VI antibody, CG6948 antibody, CLC antibody, Dmel\\CG6948 antibody, clc antibody, Clathrin heavy chain antibody, Clathrin light chain antibody, Chc antibody, Clc antibody
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