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SNAP alpha/beta 抗体

适用: 人, 大鼠, 小鼠, 斑马鱼 WB, ICC, IP 宿主: 小鼠 Monoclonal 77-2 unconjugated
产品编号 ABIN1742242
发货至: 中国
  • 抗原 See all SNAP alpha/beta products
    SNAP alpha/beta
    适用
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠, 斑马鱼
    宿主
    • 2
    • 1
    小鼠
    克隆类型
    • 2
    • 1
    单克隆
    标记
    • 3
    This SNAP alpha/beta antibody is un-conjugated
    应用范围
    Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    特异性
    Specific for alpha- and beta SNAP
    交叉反应 (详细)
    does not cross-react to gamma SNAP.
    纯化方法
    purified IgG
    免疫原
    Recombinant full length rat alpha SNAP.
    克隆位点
    77-2
    亚型
    IgG1
  • 应用备注
    WB: 1 : 1000 up to 1 : 10000 (AP staining)
    ICC: 1 : 500 up to 1 : 1000
    IHC: not tested yet
    说明

    The antibody does not immunoprecipitate the 20 S SNARE-complex.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    缓冲液
    PBS
    注意事项
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    储存条件
    -20 °C
    储存方法
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

  • 抗原
    SNAP alpha/beta
    Abstract
    SNAP alpha/beta 产品
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