HSF1 抗体 (AA 425-439)
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- 抗原 See all HSF1 抗体
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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抗原表位
- AA 425-439
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This HSF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
- 特异性
- Detects ~85 kDa (unstressed cell lysates) and ~95 kDa (heat shocked cell lysates).
- 交叉反应
- Cow, 豚鼠, Hamster, 人, 猴, 小鼠, 兔, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
- 克隆位点
- 4B4
- 亚型
- IgG1
- Top Product
- Discover our top product HSF1 Primary Antibody
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Alkaline Phosphatase (AP))HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Alkaline Phosphatase (AP)
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390
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- 应用备注
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- WB (1:1000)
- IHC (1:2000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN1741557 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- 别名
- HSF1 (HSF1 产品)
- 别名
- hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody
- 背景
- HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
- 基因ID
- 15499
- NCBI登录号
- NP_032322
- UniProt
- P38532
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