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CD14 抗体 (AA 20-214)

Name: CD14 抗体 (AA 20-214)
Brand: antibodies-online
SKU: ABIN1724926
Availability: OutOfStock

CD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated

Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red). (CD14 抗体 (AA 20-214))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD14 抗体 (AA 20-214))

ELISA

Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264. (CD14 抗体 (AA 20-214))

Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (CD14 抗体 (AA 20-214))

Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate. (CD14 抗体 (AA 20-214))

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining. (CD14 抗体 (AA 20-214))

IHC

6 images

(2 references)

产品编号 ABIN1724926

发货至: 中国

Datasheet as PDF

抗原 See all CD14 抗体

CD14
抗原表位
9

6

5

5

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-214
适用
221

37

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
人
宿主
191

52

16

3

1
小鼠
Compatible Secondaries
克隆类型
209

54
单克隆
标记
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
应用范围
196

77

55

49

45

44

20

13

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

原理

CD14 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.

克隆位点

4B4F12

亚型

IgG1
anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) antibody
CD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 适用: 人, 小鼠, 大鼠, 犬, 绵羊, 兔 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2397 unconjugated

anti-CD14 (CD14) antibody
CD14 适用: 人, Non-Human Primate FACS, WB, ELISA, IP 宿主: 小鼠 Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 适用: 人, Non-Human Primate FACS, WB, ELISA, IP, Func 宿主: 小鼠 Monoclonal MEM-18 unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Matthews, Chung, Klasse, Moore, Sanders: "Potent induction of antibody-secreting B cells by human dermal-derived CD14+ dendritic cells triggered by dual TLR ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 12, pp. 5729-44, (2012) (PubMed).

Haghparast, Heidari Kharaji, Malvandi: "Down-regulation of CD14 transcripts in human glioblastoma cell line U87 MG." in: Iranian journal of immunology : IJI, Vol. 8, Issue 2, pp. 111-9, (2011) (PubMed).
抗原

CD14

别名

CD14 (CD14 产品)

别名

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

背景

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

分子量

40kDa

基因ID

929

HGNC

929

UniProt

P08571

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades