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CD25 抗体 (AA 34-139)

Name: CD25 抗体 (AA 34-139)
Brand: antibodies-online
SKU: ABIN1724861
Availability: OutOfStock

IL2RA 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated

Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate. (CD25 抗体 (AA 34-139))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD25 抗体 (AA 34-139))

ELISA

Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green). (CD25 抗体 (AA 34-139))

Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (CD25 抗体 (AA 34-139))

Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate. (CD25 抗体 (AA 34-139))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining. (CD25 抗体 (AA 34-139))

IHC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining. (CD25 抗体 (AA 34-139))

IHC

7 images

(2 references)

产品编号 ABIN1724861

发货至: 中国

Datasheet as PDF

抗原 See all CD25 (IL2RA) 抗体

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
抗原表位
16

10

6

6

5

5

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 34-139
适用
175

110

31

9

6

3

2

2

1

1

1

1
人
宿主
129

85

77

2

1

1
小鼠
Compatible Secondaries
克隆类型
212

79

2
单克隆
标记
106

32

26

26

21

14

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is un-conjugated
应用范围
207

84

51

48

39

36

19

16

14

13

8

8

6

4

4

3

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

原理

IL2RA Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.

克隆位点

1B5D12

亚型

IgG1

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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
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应用备注

ELISA: 1/10000
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, (2012) (PubMed).

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, (2012) (PubMed).
抗原

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

别名

IL2RA (IL2RA 产品)

别名

CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

背景

Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Aliases: CD25, IL2R, TCGFR, IDDM10

分子量

30.8kDa

基因ID

3559

HGNC

3559

UniProt

P01589

途径

JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation