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Chromogranin A 抗体 (AA 87-252)

Name: Chromogranin A 抗体 (AA 87-252)
Brand: antibodies-online
SKU: ABIN1724755
Availability: OutOfStock

CHGA 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A6B6 unconjugated

Western blot analysis using CHGA mouse mAb against MOLT4 (1), SK-N-SH (2), HepG2 (3), and PC-12 (4) cell lysate. (Chromogranin A 抗体 (AA 87-252))

Western blot analysis using CHGA mAb against human CHGA recombinant protein. (Chromogranin A 抗体 (AA 87-252))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Chromogranin A 抗体 (AA 87-252))

ELISA

Flow cytometric analysis of HeLa cells using CHGA mouse mAb (green) and negative control (purple). (Chromogranin A 抗体 (AA 87-252))

FACS

Immunofluorescence analysis of HepG2 cells using CHGA mouse mAb (green). (Chromogranin A 抗体 (AA 87-252))

5 images

(2 references)

产品编号 ABIN1724755

发货至: 中国

Datasheet as PDF

抗原 See all Chromogranin A (CHGA) 抗体

Chromogranin A (CHGA)
抗原表位
21

15

7

7

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 87-252
适用
205

73

65

29

28

15

6

5

4

3

2

2

1

1
人
宿主
113

96

5

1
小鼠
Compatible Secondaries
克隆类型
122

92
单克隆
标记
92

17

11

10

10

10

10

10

7

5

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This Chromogranin A antibody is un-conjugated
应用范围
110

105

76

66

60

50

30

14

13

11

10

5

4

3

3

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

原理

CHGA Antibody

纯化方法

Ascitic fluid

免疫原

Purified recombinant fragment of human CHGA (AA: 87-252 ) expressed in E. Coli.

克隆位点

1A6B6

亚型

IgG1

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应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50

限制

仅限研究用
状态

Liquid

缓冲液

Ascitic fluid containing 0.03 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Mergler, Skrzypski, Sassek, Pietrzak, Pucci, Wiedenmann, Strowski: "Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells." in: Cellular signalling, Vol. 24, Issue 1, pp. 233-46, (2011) (PubMed).

El Ali, Fichna, Piniewska, Kosowicz, Grzymis?awski: "Chromogranin A as a useful neuroendocrine marker in patients with autoimmune Addison's disease." in: Journal of endocrinological investigation, Vol. 33, Issue 3, pp. 186-91, (2010) (PubMed).
抗原

Chromogranin A (CHGA)

别名

CHGA (CHGA 产品)

别名

CGA antibody, CG-ALPHA antibody, FSHA antibody, GPHA1 antibody, GPHa antibody, HCG antibody, LHA antibody, TSHA antibody, ChrA antibody, fj05f09 antibody, si:dkey-177p2.2 antibody, wu:fj05f09 antibody, zgc:101749 antibody, zgc:56075 antibody, CHGA antibody, cgA antibody, chga antibody, chromogranin A antibody, glycoprotein hormones, alpha polypeptide antibody, uncharacterized CHGA antibody, chromogranin A S homeolog antibody, CHGA antibody, CGA antibody, Chga antibody, chga antibody, chga.S antibody

背景

Description: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.

Aliases: CGA

分子量

50.7kDa

基因ID

1113

HGNC

1113

UniProt

P10645

途径

Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling