(2 references)-
- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HRP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 免疫原
- Purified recombinant fragment of HRP expressed in E. Coli.
- 克隆位点
- 3A5C6
- 亚型
- IgG1
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish WB, ELISA, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
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Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Ascitic fluid containing 0.03 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4 °C or at -20 °C for long term.
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: "Time course of angiogenesis in solid pineal autografts." in: Cell and tissue research, Vol. 315, Issue 3, pp. 349-59, (2004) (PubMed).
: "Peroxidase-catalyzed co-oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of substituted phenols and their polydisulfides." in: Journal of inorganic biochemistry, Vol. 98, Issue 1, pp. 1-9, (2003) (PubMed).
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: "Time course of angiogenesis in solid pineal autografts." in: Cell and tissue research, Vol. 315, Issue 3, pp. 349-59, (2004) (PubMed).
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- HRP (HRP 产品)
- 背景
- Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
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