D1-Nt 抗体
PsbA
适用: Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum
WB
宿主: 兔
Polyclonal
unconjugated
1 image
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- 抗原
- D1-Nt (PsbA)
- 适用
- Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 特异性
- Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.
- 免疫原
- synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.
- 亚型
- IgG
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- 应用备注
- Western blot (1 : 2.000 for ECL)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C
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- 抗原
- D1-Nt (PsbA)
- 背景
- D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).
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