Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
交叉反应 (详细)
Cross reactivity with PARL (presenilin associated, rhomboid-like) from other sources has not been determined.
纯度
Anti-PARL was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards PARL. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Chicken, and Primate based on 100 % sequence homology.
内毒素水平
Low Endotoxin : No
免疫原
PARL affinity purified antibody was prepared from whole rabbit serum produced by repeated immunization from a synthetic peptide corresponding to the C terminus region of human PARL. Immunogen Type: Peptide
Anti-PARL antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42.2 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 1 µg/mL
限制
仅限研究用
状态
Liquid
浓度
0.86 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
储存条件
4 °C/-20 °C
储存方法
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
有效期
Expiration date is six (6) months from date of opening.
PARL (presenilin associated, rhomboid-like) encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. Anti-PARL antibodies are ideal for researchers interested in Apoptosis, Alzheimers Research, Neuroscience, and Mitochondrial Fusion Proteins research. Synonyms: EC 3.4.21.105, mitochondrial, presenilin associated, rhomboid-like, PRO2207, rhomboid 7 homolog 1